McKinnon starred in the Logo sketch comedy series The Big Gay Sketch Show (2007–2010), voiced lead roles in the PBS Kids animated series Nature Cat (2015–present) and the Netflix animated series The Magic School Bus Rides Again (2017–2021), and portrayed Carole Baskin in the Peacock miniseries Joe vs. Carole (2022).
McKinnon has also appeared in numerous films, such as Balls Out (2014), Ghostbusters (2016), Office Christmas Party (2016), Rough Night (2017), The Spy Who Dumped Me (2018), Yesterday (2019), Bombshell (2019), DC League of Super-Pets (2022), and Barbie (2023).
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