Marino began his career as a cast member on MTV's sketch comedy series The State from 1993 to 1995. He has since starred in a number of television shows, including Party Down (2009-2010), Marry Me (2014-2015), Burning Love (2012-2013), and Childrens Hospital (2008-2016). He has also appeared in films such as Wet Hot American Summer (2001), The Ten (2007), Wanderlust (2012), and Brooklyn Nine-Nine (2013-2021).
Marino is married to actress Erica Oyama, and they have two children together. He is a frequent collaborator with his friend and fellow actor David Wain, and they have worked together on a number of projects, including The State, Wet Hot American Summer, and Childrens Hospital.